- Title
- Compositional and functional differences of the mucosal microbiota along the intestine of healthy individuals
- Creator
- Vaga, Stefania; Lee, Sunjae; Ji, Boyang; Andreasson, Anna; Talley, Nicholas J.; Agréus, Lars; Bidkhori, Gholamreza; Kovatcheva-Datchary, Petia; Park, Junseok; Lee, Doheon; Proctor, Gordon; Ehrlich, Stanislav Dusko; Nielsen, Jens; Engstrand, Lars; Shoaie, Saeed
- Relation
- NHMRC
- Relation
- Scientific Reports Vol. 10, Issue 1, no. 14977
- Publisher Link
- http://dx.doi.org/10.1038/s41598-020-71939-2
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2020
- Description
- Gut mucosal microbes evolved closest to the host, developing specialized local communities. There is, however, insufficient knowledge of these communities as most studies have employed sequencing technologies to investigate faecal microbiota only. This work used shotgun metagenomics of mucosal biopsies to explore the microbial communities' compositions of terminal ileum and large intestine in 5 healthy individuals. Functional annotations and genome-scale metabolic modelling of selected species were then employed to identify local functional enrichments. While faecal metagenomics provided a good approximation of the average gut mucosal microbiome composition, mucosal biopsies allowed detecting the subtle variations of local microbial communities. Given their significant enrichment in the mucosal microbiota, we highlight the roles of Bacteroides species and describe the antimicrobial resistance biogeography along the intestine. We also detail which species, at which locations, are involved with the tryptophan/indole pathway, whose malfunctioning has been linked to pathologies including inflammatory bowel disease. Our study thus provides invaluable resources for investigating mechanisms connecting gut microbiota and host pathophysiology.
- Subject
- metagenomics; microbiome; mucosal; inflammatory bowel disease; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1430703
- Identifier
- uon:38873
- Identifier
- ISSN:2045-2322
- Rights
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
- Language
- eng
- Full Text
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